At Day 10, the reactive callus (HE), cartilaginous fraction (Alcian blue), as well as early woven bone (Movat pentachrome) was quantified. Movat's stain is a pentachrome stain originally developed by Henry Zoltan Movat (1923–1995), a Hungarian-Canadian Pathologist in Toronto in 1955 to highlight the various constituents of connective tissue, especially cardiovascular tissue, by five colors in a single stained slide. Longitudinal sections (8 μm) using the hole region as landmark were cut and stained for H&E, aniline blue, TRAP, movat pentachrome, or Safranin O. Fluorescent Silica Nanoparticles to Label Metastatic Tumor Cells in Mineralized Bone Microenvironments [published online ahead of print, 2020 May 28]. (D) Movat pentachrome staining of fetal bone marks three distinct regions within the bone: (i) Hypertrophic zone, (ii) Growth plate and (iii) Ossification zone. Movat Pentachrome Stain Kit (Modified Russell-Movat): The Movat Pentachrome Stain Kit (Modified Movat) is intended for use in histological demonstration of collagen, elastin ... Wright-Giemsa Stain Kit is intended to be used for differential staining of blood smears, bone marrow and blood parasites. Positive staining is stria, type II fibrocytes of … The control group shows the physiological healing pattern (1) while group D4 (2) and group D7 (3) are characterized by prominent haematoma remnants and a delayed periosteal callus formation (overview top). (A) Red fluorescence in tibiae (top panels), μCT images (middle panels), and representative Movat-Pentachrome stains (lower panels; yellow, mineralized bone; red, new bone; blue, cartilage; purple, nuclei) of fracture calluses 14 days after fracture and intratibial injection of the indicated cell populations. It is a mix of 5 stains that are used to highlight the various constituents of connective tissue especially in the lung, developing/healing bone and cardiovascular tissue. Proximal cortical bone and newly formed bone closing the medullary cavity in an empty 30‐mm defect, after 3 months, visualized with Movat Pentachrome stain (A–C), Picrosirius Red and polarized light microscopy (D–F), and backscattered electron imaging (G–I). The Movat–Pentachrome staining was performed for histomorphometry . (A) Red fluorescence in tibiae (top panels), μCT images (middle panels), and representative Movat-Pentachrome stains (lower panels; yellow, mineralized bone; red, new bone; blue, cartilage; purple, nuclei) of fracture calluses 14 days after fracture and intratibial injection of the indicated cell populations. Movat’s pentachrome staining . Histopathologist’s Palette 2: The Movat Pentachrome Edition ️. Bone is a complex organ with the capacity to regenerate. THE FISCHBACH LAB (2018) ... Hinckley JA, Khaitan R, et al. Movat’s Pentachrome stain, Normal, L 247 Home / Temporal Bone Image Library / Movat’s Pentachrome stain, Normal, L 247 Title: Movat’s Pentachrome stain, Normal, L 247 Movat pentachrome, CD68, α-SMA and osteocalcin immunostaining of decalcified bone sections were performed as described in [14, 15, 19, 20]. High-resolution Movat’s pentachrome stain of the ramus in the acellular scaffold group. 5C, D). To analyze whether absence of lymphocytes influences bone formation, osteoblast number in the bony callus was determined on days 14 and 21 in Movat‐pentachrome‐stained tissue (Fig. Micro-CT scan. Fig. Scale bar indicates 5 mm. Fig. To quantify kidney grafts, analytical software (Adobe Photoshop CS6) was used to measure pixel area of engrafted bone … Whisker indicates minimum and maximum. At Day 28 in both groups, a prominent callus was visible consisting of mineralized woven bone adjacent to the cortex and cartilage and connective tissue within the osteotomy gap. After embedding in methyl methacrylate, this procedure yields consistently good results, with an excellent and colorful contrast between mineralized and unmineralized compartments of both cartilage and bone. (Movat–Pentachrome staining). The intramembranous areas of the callus at day 14 were analyzed (n = 8) using a computer aided method to determine the areas between the newly formed woven bone in order to determine the porosity. Small. The femur-graft-tibia complex (n = 5 from each group at each time point) were stored at -80°C immediately after collection for subsequent micro-CT scanning. ... Movat pentachrome; and Ab, Alcian blue on paraffin- or plastic-embedded sections of long bone samples of mouse and sheep. Qualitative analyses are also conducted through immunohistochemistry, Tetrate-resistant acid phosphatase (TRAP) stain and Russell-Movat pentachrome stain. Immunostaining of collagen I and II … Even with this healing potential, healing results in fractured bone are unsatisfactory in a considerable patient cohort even with a good treatment regimen. MOVAT'S PENTACHROME STAIN OF THE GROWTH PLATE IN A MURINE PROXIMAL TIBIA. RUSSELL MODIFICATION OF MOVAT PENTACHROME STAIN • REAGENTS: Alcian blue - 20 min Heamtoxylin - 15 min 2% aq.ferric chloride - differentiation Crocein – sacrlet acid fuschin -5 min Phototungstic acid – 5 min Alcoholic sufran - 15 min • RESULTS: Elastic fibres - black Collagen and reticular fibres – yellow Muscle - red 36 37. •Macrophages were stained and investigated using an antibody against CD68. Movat Pentachrome staining was performed to evaluate the relative amount of mineralized bone, cartilage, connective tissue and bone marrow . In Figure 10A , representative Movat Pentachrome pictures are presented to illustrate the tissue formation in and around the osteotomy gap at the respective time point. Immunofluorescent (IF) images showing the spatial organization of hSSC markers in the longitudinal bone … Movat's pentachrome I stain has been adapted and modified as a stain for Undecalcified bone sections. Grafts were then cryosectioned at 10 μm and stained by Movat Pentachrome Staining Kit (ScyTek, MPS-1) to demonstrate bone and cartilage differentiation. At the later time points, Days 28–84, the amount of mineralized and cartilaginous tissue (Safranin orange/van Kossa) was evaluated. stained for Movat’s pentachrome showing the different composition of tissue formed within the 3 cm defects Figure 25: High magnification images of PMMA sections stained for Movat’s pentachrome illustrating the different degrees of bone maturation within the different experimental groups The SEM sample is an axial section showing a few colonized samples (corresponding to Figure 3 [5a]) in which new bone formation shows typically woven bone characteristics (gray) within the macropores of the ceramics (Cer) (white). High-resolution Movat’s pentachrome stain of the ramus in the tissue-engineered bone … Modified Movat’s stain is a stain developed by Henry Zoltan Movat in 1955. Statistical analysis was done with Bias 11.02 (Epsilon-Verlag, Darmstadt, Germany). Differences in osteoblast numbers on day 14 suggested higher … S9. S8. •The intramembranous areas of the callus at day 14 were analyzed (n = 8) using a computer aided method to determine the areas between the newly formed woven bone in order to determine the porosity. CANCER-ASSOCIATED ADIPOCYTES ON FIBRONECTIN. References . Scanning electron microscopy (SEM) and selected areas stained with Movat pentachrome (right panels). Arch Path., 60:289-295, 1955. Among the three experimental groups, the tissue-engineered grafts most strongly enhanced the graft-host bone integration. High-resolution Movat’s pentachrome stain of the condyle in the condylectomy group at 6 months. Finally, histological staining through Movat Pentachrome determine the tissue types present within the gap of the four groups. Bone Section from a methyl-methacrylate block in a Movat Pentachrome stain : Cross section from a polyphenylensulphide material with a high amount of carbon fibres : Human Prostate Gland Section : to be continued. in addition, osteoblats, osteoclasts, and other cells and tissue components can … (B) Movat pentachrome stain classified image depicts ossified tissue as red, osteoid (non-mineralized) as green, cartilage as magenta, bone marrow as yellow, and background class as turquoise. Movat’s pentachrome showed positive red staining of bone tissue and osteoid tissue in decalcified sections. Paired histological (Movat’s pentachrome stain) and μCT image analysis distinguished the implanted scaffold material (both acellular and cell-laden) from the native bone . Representative sections were stained with Movat’s Pentachrome stain . original Movat ’ s pentachrome staining (Movat 1955) and its later modi cations (Clifford and T aylor 1973, Garvey et al. Movat, H.Z .,: Demonstation of all C onnecti ve Tissue Elements in a Single S ection. 1986, Russell 1972, Schmidt and Wirtala Movat Pentachrome staining 14 days post surgery of the control group and the haematoma removal groups (D4, D7). After 3 weeks of healing, OI-iPSC constructs were still … Title: Immunostaining, Na K ATPase Chapter: Removal & Study of Temporal Bones Chapter Section: Temporal Bone Removal and Preparation Comments: Newborn male PM time 2 hours, embedded in polyester wax. Scale: 500 μm (left). Histological analysis (Movat pentachrome staining) revealed accelerated callus maturation in the BMP-2 group (Figure 3), which supported previous μCT data. Mature bone is shown in a compact structure in dark yellow compared with fibrous tissue in light … The Movat’s pentachrome staining presented one of the best coloring methods for detailed analysis of bone healing progressions. Upon quantitative analysis, the bone volume per tissue volume (BV/TV) (***P<0.001) and callus volume (CV) (****P<0.0001) were significantly higher in the distraction group compared to the sham group (Figure 1C). H&E staining and Russell-Movat pentachrome staining were performed to evaluate osteogenesis at the tendon-bone interface using light microscopy. Fig. Roman N., et al: Orcein-Hematoxylin in I … The power analysis was planned to detect differences in histological evaluation of bone mass increase (based on Movat's pentachrome staining) between four experimental groups (syngenic bone, small, medium, large granules). Comparisons between Vegfa CKO and control mice were primarily covering the hole region between the interrupted cortical bone ends and adjacent areas, visible in the same sections. S7. As the healing period increased, the formation of new bone increased and the amount of residual graft …

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