Fresh whole blood films, bone marrow films, or specimens anticoagulated in EDTA should be used. 2. Specimens of bone marrow trephine biopsy (BMT) are transported and fixed in acetic acid–zinc–formalin fixative, decalcified in 10% formic acid–5% formaldehyde and processed with other specimens to paraffin-wax embedding. For patient safety and convenience, biopsies are usually performed on the posterior iliac crest. The remarkable property of the Romanowsky dyes of making subtle distinctions in shades of staining, and of staining granules differentially, depends on two components: azure B (trimethylthionine) and eosin Y (tetrabromofluorescein). A bone marrow aspiration should be performed as part of the same procedure. Bone Marrow Trephine Biopsy Specimens B. Preparing bone marrow smears using non-anticoagulated bone marrow aspirate is a traditional practice but many laboratories now use anticoagulated aspirate samples in K-EDTA. in the adult, this can also be identified as either red or yellow marrow. 2. The Bone Marrow Aspirate 2.1 Collection of Aspirate Specimens 2.2 Preparation of Aspirate Slides 2.3 Preparation of Particle Clots 2.4 Staining of Bone Marrow Aspirate Slides 2.5 Microscopy 2.6 The Nucleated Differential Count 2.7 Storage Iron 2.8 Supplementary Investigations 2.9 The Aspirate Report 3. Bone marrow aspirate smears were stained with May-Grunwald Giemsa stain and biopsy sections were stained with hematoxylin and eosin as per standard protocols. The quality of the marrow sections is governed by numerous variables related to specimen collection and processing and must be considered. Replaces woven bone. The staining procedures listed in this insert have given satisfactory results in our laboratory. Many collagen fibers parallel to each other in the same layer. Giemsa stain is a classic blood film stain for peripheral blood smears and bone marrow specimens. Sections, 1-μm-thick, are cut by experienced histotechnologists and used for haematoxylin and eosin, Giemsa, reticulin silver and other histological stains. Staining blood and bone marrow films . Trephine biopsies of the bone marrow should be carried out, when clinically indicated, by trained individuals following a standard operating procedure. Definition Hematopoiesis is the proliferation of progenitor cells, which are maintained by the stem cells and their differentiation into all the cellular components of blood. BONE MARROW EXAMINATION TEST DESCRIPTIONS: Bone marrow examinations may include aspiration with smears, particle preparations or clot sections and biopsies. I. Romanowsky stains are used universally for routine staining of blood films. NOTES: 1. INTRODUCTION A. Red Blood Cells stain pink, platelets stain a light pale pink, lymphocyte cytoplasm stains sky blue, monocyte cytoplasm stains pale blue, and leukocyte nuclear chromatin stains magenta. Individual color preferences may necessitate slight adjustments to the times utilized. 1. Aspirate smears are most useful to examine the cytological detail of the marrow elements and for cytochemical and immunocytochemical stains. Sites of hematopoiesis depend on the presence of disease and on the develop-mental state of the individual. (2) In the bone marrow in slide 48, look for marrow sinuses and cords View Image, which are expanded sinusoidal (discontinuous) capillaries characteristic of marrow. In addition to discussing normal structure, function, and histology of bone marrow, methods for preparation and evaluation of bone marrow are presented. medullary cavity - (bone marrow) refers to the cavity within the bone, that is lined with cells (endosteum) and filled with bone marrow. 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